目的 建立高效液相色谱法同时测定25批蜘蛛香中3-O-咖啡酰基奎宁酸、橙皮苷和4,5-O-二咖啡酰基奎宁酸含量的方法。方法 以Phenomenex Gemini C18(4.6 mm×250 mm,5 μm)为色谱柱,乙腈(A)-0.1%磷酸水溶液(B)为流动相系统梯度洗脱,流速为1.0 mLmin-1,检测波长340 nm,柱温25 ℃,进样量10 μL。运用该方法对25批蜘蛛香药材进行测定,并通过聚类分析对结果进行分析。结果 蜘蛛香中3-O-咖啡酰基奎宁酸、橙皮苷和4,5-O-二咖啡酰基奎宁酸的线性范围分别为0.33~1.97 μg(r=0.999 9), 1.14~6.84 μg(r=0.999 9), 0.11~0.66 μg(r=0.999 9), 平均加样回收率(n=6) 分别为97.02%(RSD 0.6%), 100.60%(RSD 1.7%), 96.79%(RSD 1.0%)。采用聚类分析能将不同产地的25批蜘蛛香药材分成3类。结论 该方法简便、快速、准确,可用于蜘蛛香药材的质量评价和控制。
Abstract
OBJECTIVE To establish a method for simultaneous determination of three components, namely, 3-O-caffeoylquinic acid, hesperidin and 4,5-O-dicaffeoylquinic acid in Valeriana jatamansi. METHODS A Phenomenex Gemini C18 column (4.6 mm×250 mm,5 μm) was used with mobile phase consisting of acetonitrile-water(containing 0.1% phosphoric acid) gradiently eluted at a flow rate of 1.0 mLmin-1. UV detection wavelength was set at 340 nm, the column temperature was maintained at 25 ℃, and the sample injection volume was 10 μL. The three components from 25 batches of Valeriana jatamansi were determined by HPLC in combination with cluster analysis. RESUTLS The liner ranges of 3-O-caffeoylquinic acid, hesperidin and 4,5-O-dicaffeoylquinic acid were 0.33-1.97 μg(r=0.999 9), 1.14-6.84 μg(r=0.999 9), 0.11-0.66 μg (r=0.999 9), respectively. The average recoveries(n=6) were 97.02%, 100.60%, and 96.79%, with RSD of 0.6%, 1.7%, and 1.0%, respectively. The 25 batches of Valeriana jatamansi were divided by cluster analysis into three groups according to the total amount of the three chemical markers. XONCLUSION The established HPLC method is simple, rapid and accurate, and can be used to control the quality of Valeriana jatamansi.
关键词
高效液相色谱法 /
蜘蛛香 /
3-O-咖啡酰基奎宁酸 /
橙皮苷 /
4 /
5-O-二咖啡酰基奎宁酸 /
聚类分析
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Key words
HPLC /
Valeriana atamansi /
3-O-caffeoylquinic acid /
hesperidin /
4,5-O-dicaffeoylquinic acid /
cluster analysis
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中图分类号:
R917
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参考文献
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脚注
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基金
国家科技支撑计划课题(2013BAI11B01);贵州省中药现代化科技产业研究开发专项(黔科合重G字[2013]4001);贵州省高等学校创新能力提升计划(黔教合协同创新字[2013]04)
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